Centaurea uysalii Şirin & Çeçen, a new Asteraceae species from the province of Karaman, in Turkey, is described and illustrated herein. It is morphologically similar to C. bourgaei Boiss. (Subgen. Cyanus), but it mainly differs from its relative in terms of the involucre and achene lengths. The achene micromorphological features and karyomorphology of C. uysalii and C. bourgaei were examined in this study. The IUCN conservation status is the Critically Endangered [CR B2 a b (i, iii)] category.