Boron (B) is an essential micronutrient required for normal plant growth and development. Boron management is challenging because the optimum B application range is narrow and the application rates vary from one soil to another. Boron deficiency is widespread in the Anatolia region of Turkey. This may impact on yield and mineral contents of leaves and fruits of strawberry (Fragaria × ananassa cv. Fern) especially in B-deficient calcareous Aridisols in Eastern Anatolia, Turkey. A 2-year field experiment was conducted to study yield and quality response of strawberry to B application. Boron fertilizer application affected plant yield and chemical composition. B application decreased tissue nitrogen (N) and calcium (Ca) but increased tissue phosphorus (P), potassium (K), manganese (Mn), zinc (Zn) and copper (Cu) content. We conclude that a B addition of 5.5 kg ha-1 is sufficient to elevate soil B levels to non-deficient levels. © 2011 Taylor & Francis.