Background: The aim of the present study was to establish the normal ranges for foetal nasal bone length (NBL), prenasal skin thickness (PNT), interocular distance (IOD), and ratio of prenasal thickness to- nasal bone length (PNT/ NBL) at 18-24weeks using two-dimensional (2D) ultrasound. Methods: This study was a retrospective study of prenatal ultrasonographic records from 407 foetuses between 18 and 24weeks gestational age (GA). The NBL, PNT, IOD, PNT/ NBL ratio, biparietal diameter (BPD), and femur length (FL) were investigated. The relationships among NBL, PNT, IOD, PNT/ NBL, and GA were evaluated. Additionally, descriptive statistics for NBL, PNT, and IOD values for each gestational week were obtained. Results: There was a significant association between GA and NBL, PNT, and IOD between 18 and 24weeks. NBL increased from a mean of 5.5mm to 8.3mm, PNT increased from a mean of 3.5mm to 5.1mm, and IOD increased from a mean of 11.1mm to 14.5mm. PNT/NBL ratio did not change with gestational age. Conclusions: This study showed normal ranges for NBL, PNT, IOD, and PNT/ NBL ratios for foetuses between 18 and 24weeks in low-risk pregnancies. There was a positive linear relationship between GA and NBL, PNT, and IOD. The PNT/NBL ratio might be a more useful measurement than NBL or PNT alone.