This study aimed at determining the total losses associated with the season, transportation distance, and slaughter age during the transportation of broilers from poultry farms to slaughterhouses in Turkey. All data and parameters were statistically analyzed and the change in total loss during transportation was evaluated by two-way analysis of variance to determine which factors or variables affect this change. Total transportation losses were compared among the four seasons of the year, two slaughter ages (younger broiler, 31-39 days of age; older broilers 40-46 days of age) and three distance ranges (short, ≤50 km; medium, 51-150 km; and long, ≥151 km). Total losses of 259.40 g, 307.35 g, and 350.14 g were determined for short, medium and long distances, respectively, indicating that losses increased with transportation distance (p<0.05). Broilers slaughtered at a younger age presented lower total losses than those slaughtered at an older age (p<0.05). When seasons were evaluated, the highest total loss was determined in the summer, which was not statistically different from that calculated for autumn, whereas total transportation losses in spring and winter were found relatively lower. The study showed long distance transportation in the winter considerably increased total losses to levels similar to those obtained in the summer.