The aim of this study is to determine the importance of B-mode ultrasonography in the diagnosis of hepatic lipidosis in dairy cows and compare this mode of diagnosis with both the histologic examination of liver biopsy samples and investigation of some biochemical parameters associated with hepatic lipidosis. 15 Holstein cows with moderate hepatic lipidosis and 15 cows with severe hepatic lipidosis and 6 healtyh cows were used as a metarilas. Blood samples were obtained from all cows and analyzed. Liver samples were obtained by biopsy in cattle. The ultrasonographic examination of liver was performed on animal. Serum glutamate dehydrogenase (GDH) and aspartate amino transferase (AST) concentrations were increased in cows with moderate hepatic lipidosis. Serum sorbitol dehydrogenase (SDH), GDH, and AST concentrations were increased in cows with severe hepatic lipidosis. Ultrasonographic examination revealed an increase in diffuse echogenicity of the liver in cows with moderate and severe hepatic lipidosis cows, but the increase was little in moderate hepatic lipidosis. Both serum GDH and SDH levels were found to be increased in severe hepatic lipidosis. However, only the serum GDH level was elevated in moderate hepatic lipidosis. Therefore, both ultrasonographic examination and measurement of specific liver enzymes seem to be beneficial in the diagnosis of hepatic lipidosis.