The natural occurrence of aflatoxin was investigated in layer feed and corn samples brought to Konya Veterinary Control and Research Institute Laboratory between 15 April and 15 December 2002. Seventy-eight samples (52 feeds, 26 corn samples) were analysed for total aflatoxin (B1 + B2 + G1 + G2) by an ELISA screening method. Aflatoxin contamination was determined in 37 feed samples (71.1%) and 15 corn samples (57.7%), with a range of 1.5-133 μg kg-1. However, a majority of the aflatoxin contamination was less than 5 μg kg-1 (50% within the positive samples). Two feed samples and two corn samples exceeded the maximum tolerated levels in feed (20 μg kg-1) and feedstuffs (50 μg kg-1) for total flatoxin.