Objectives: To investigate the ideal time and reproducibility of anterior chamber flare measurements. Materials and Methods: Anterior chamber flare measurements were performed with laser flaremetre device at 8 am to 45 volunteers and these measurements were repeated on the same day at 12 pm and 4 pm. Results: Twenty-five (55.5%) of the volunteers were women and 20 (44.5%) were men; mean age was 28.67±7.40 (18-49) years. The mean anterior chamber flare measurements taken following the ophthalmologic examination were 5.94±1.41 foton/msn at 8 am, 5.65±1.45 foton/msn at 12 pm, and 5.79±1.20 foton/msn at 4 pm. No statistical difference was found between the measurements (p = 0.08). Subgroup analysis according to eye color, revealed no significant difference between flare measurements in brown, hazel, and green eyes (p = 0.21). Correlation analysis demonstrated association between age and all flare measurements within the day (r = 0.24, p=0.03; r=0.4l, p = 0.01, r=0.27, p = 0.01). Conclusion: No significant diurnal change was detected in the flare measurements of our study subjects but positive correlation with age was observed. Hence, all flare measurements within a day are reliable and have high repeatability in healthy subjects.