The purpose of this study was to determine the structural and functional properties of the distal muscles of front and hind legs of Malakan Horses. Thus, totally 10 Malakan horses (5 females and 5 males) were used in the study. From front and hind legs of the horses, the findings of totally 28 muscles were received. Accordingly, it is determined that the longest muscle in the distal of the front leg was m. flexor carpi ulnaris, the heaviest one was m. extensor carpi radialis and the muscle with the highest value in terms of parameters of tendon length and total muscle tendon length was m. extensor digitorum communis. From the distal muscles of the hind leg; it is observed that the longest one was m. tibialis cranialis, the heaviest one was m. gastrocnemius and the muscle with the highest value in terms of parameters of tendon length and total muscle tendon length was m. flexor digitorum superficialis. It was observed that while the difference determined between some of the values in the front and hind leg muscles had a statistical significance in the comparison performed between the genders (P<0.05), the differences had no statistical significance in the comparison performed based on the direction (right-left).