In this study, a total of 254 blood sera samples taken from cattle of different age, sex and breed at Meat and Fish Association Slaughter House in Konya were tested against neutralizing antibodies for Bovine Adenovirus type 1, 2 and 3 (BAV-1, 2 and 3), Infectious Bovine Rhinotracheitis / Infectious Pustular Vulvovaginitis Virus (IBR/IPV), Parainfluenza type 3 (PI-3) Virus, Bovine Respiratory Syncytial Virus (BRSV) and Bovine Viral Diarrhea Virus (BVDV) by microneutralization test (mNT). At the end of the serological control by mNT, neutralizing antibody presence was detected against BAV-1 in 56 (22.04%), BAV-2 in 38 (14.96%), BAV-3 in 51 (20.07%), PI-3 in 137 (53.93%), IBR/IPV in 145 (57.08%), BRSV in 117 (46.06%) and BVDV in 112 (44.09%) animals. In the cattle tested for antibodies, 14.7% were positive for only one virus, 36.22% of sera had antibodies to two viruses, 29.92% of sera had antibodies to three viruses, 14.56% of sera had antibodies to four viruses, 3.93% of sera had antibodies to five viruses, 1.57% of sera had antibodies to six viruses and 0.39% of sera had antibodies to seven viruses. However antibodies were not detected in 3.15% of the 254 sera.