In this study, a controlled fuzzy expert system (FES) was designed to provide the conditions necessary for operating rooms. For this purpose, existing operating rooms have been studied to see if there are more useful, reliable and comfortable ones. How an operating room can be controlled with FES and its advantages and disadvantages have also been researched. For a theoretically visible FES to show system's advantage a prototype operating room was built and a suitable configuration was designed. In this system, heat, humidity, oxygen and particles were used as input parameters, and a fresh air entrance and fan circulation were chosen as output parameters. With the help of an expert, appropriate linguistic expressions and the membership function of these expressions were defined. The sensors were classified and sensor information was transferred to computer by means of an interface designed. In order to transfer the data to the system simultaneously, an interface was written in C#. Whether it provides the most suitable control for the system prototype was determined by simulating the operation with varying numbers of patients and operation personnel. In these trials, input, output and other necessary parameters were collected in the computer. In the study, we obtained excellent results in prototype operating room control with FES. The analyses of the results carried out indicated that the controls performed with FES provide more economical, comfortable, reliable and consistent controls and that they are feasible in a real operating room. © 2009 Elsevier Ltd. All rights reserved.