Biodiesel is an alternative fuel with catalyst and alcohol reaction production from vegetable oils, animal oils, and waste fried oils. Biodiesel is the primary environmentally friendly, renewable diesel fuel. Storage time and conditions of biodiesel effect flow properties of biodiesel in low temperatures are some of the problems that need to be solved before using biodiesel in diesel engines. In this study, physical and chemical properties of safflower methyl ester and palm methyl ester produced by transesterification methods are measured. The variation of viscosity with a range of temperature of 25-90°C is investigated during storage and in different conditions. This study is aimed at investigating the effects of storage conditions on viscosity of biodiesel. As a result, density (kg/m3), viscosity (40°C, mm2/s), flash point (°C), Cu corrosion test, Cetane number, cloud point (°C), flow point (°C), freezing point (°C), and heating values (MJ/kg) are, respectively, in the SME 880, 4.029, 180, 1a, 49.8, -4, -6, -11, 40.0, in the PME 880, 6.662, 90, 1a, 56, 9, 6, 5, 39.25 have determinate.