In this research, it was aimed to determine the effect of eugenol with concentrations of 0.5% and 1.0% on L. monocytogenes with amount of 103 cfu/g, 104 cfu/g, 105 cfu/g and on total aerobic mesophilic microorganisms. In the experimental samples, presence of L. monocytogenes, the total aerobic mesophilic microorganisms and pH values were determined in the beginning (day 0), 5th and 10th days of cold storage at 4°C and 30th and 60th days of frozen storage at -18°C. There were significant differences in the number of total aerobic mesophilic microorganisms at the beginning day (day 0) and 30th day of the experimental Inegöl meatballs between groups, statistically (P<0.01). Considering the storage period, it was observed that the number of initial total aerobic mesophilic microorganisms was reduced in experimental samples during whole storage time (P<0.01). As an overall evaluation it was determined that the initial number of L. monocytogenes untreated with eugenol was higher than the samples treated with eugenol 0.5% and 1% after 60 days of frozen storage. This difference probably indicates that eugenol has an inhibitory effect on L. monocytogenes.