In experimental researchs using animal in Turkey, the person who will do practice on animal will have to get “Certificate of Experimental Animal Use” has come into force by “Animal Experiments Regulation on Working Procedures and Principles of the Ethics Committees”. According to this regulation it is reported that related person will be given a training consisting of up to 80 hours. It is aimed to evaluate that researches who attended in animal experiments research training program the attitudes towards training activities and use of animals in experiments. The material of the study is consisted of 208 participants who are from different cities and attended different training program between 2012 to 2015. In the study, participants were given a questionnaire consisting of two sections. Surveys to determine the course of six demographic questions and attitudes towards the use of animals in experiments with activity “Likert-type” prepared 14 questions were asked. The data were collected through questionnaires frequency distributions using SPSS 20.0 statistical software were determined. The data were subjected to t-test and analysis of variance test, P-value <0.05 was considered as a significant. In this research, 74% of respondents (n = 154) reported, in terms of accountability in the use of animals “Animal Production and/or Hosting Unit” in favor of the requirements. A significant difference in terms of gender in the use of animals for the benefit of people with the priority of human interests were found. Courses that require training (87%, n = 181) and will contribute to the conscious animal use in research (87.5%, n = 182) were found to be effective against this view of the experience of working with educational status. Consequently, the legal regulations issued in terms of monitoring the use of animals has been accepted by the investigators; although human-centered approach is present, the use of experimental animals can be said that given a more acceptable form through adequate supervision and awareness-raising by education.