All diesel engines work with a specially designed injection system. The lubrication of these moving inner parts is achieved with fuel. The inadequacy of lubrication properties of the fuel either causes low pressure or wear. Applications carried out to decrease the sulfur level to lower than 10 ppm have also been effective in decreasing particulate emissions. However, this process decreases the lubrication properties of the fuel. As a solution to this problem, instead of diesel, biodiesel, which has comparatively better lubrication properties, is suggested. In this study, the physical and chemical properties of biodiesels produced from safflower oil, cottonseed oil, soybean oil, and sunflower oil are investigated and then their lubrication performances were investigated with a pin-on-disc test device under constant load with different sliding velocities. The results obtained were compared with euro-diesel. The coefficient of friction was the lowest in safflower oil biodiesel, which was followed by cottonseed oil biodiesel, soybean oil biodiesel, sunflower oil biodiesel; the highest coefficient of friction was for euro-diesel. Copyright © Society of Tribologists and Lubrication Engineers.