Abstract This study presents the preparation of gold nanoparticle functionalized graphene oxide (AuNPs-GO) as a new bio-compatible material for sensor applications. The electrocatalytic activity of AuNPs-GO towards electrooxidation of H2O2 was also described by using electrochemical techniques. The fabricated biosensor showed a linear range from 0.1 mM to 3.8 mM with a sensitivity of 17.68 μA mM-1 cm-2 and a detection limit of 0.075 mM for the detection of glucose.