Candida rugosa lipase has been immobilized on a variety of calix[4]arene derivatives using the sol-gel encapsulation technique, and the catalytic activities of the resulting encapsulated lipases towards the hydrolysis of pnitrophenyl palmitate and the hydrolytic kinetic resolution of racemic Naproxen methyl ester were evaluated using standard techniques. The results revealed that the calix[4]- arene-based immobilized encapsulated lipases 5-CRL and 6-CRL gave higher levels of enantioselectivity and conversion than the free encapsulated lipase.